Genotyping-by-sequencing (GBS)
This is a simultaneous genotyping and SNP discovery method that can be tailored to suit your species genotyping requirements. GenomNZ employs both restriction enzyme-based and targeted sequencing GBS methods utilising next-generation sequencing technologies.
For restriction enzyme-based GBS, combining low-depth sequencing with algorithms that produce bias-free genomic relationship matrices we can estimate: breed composition, pedigree, traceability, inbreeding and co-ancestry as well as using directly in existing mixed models (GBLUP) to estimate breeding values. In addition, further developments for GBS analysis has established methods to undertake genome wise association analyses, linkage mapping, estimation of linkage disequilibrium and derivatives such as Ne.
GenomNZ uses this technology for the genotyping of deer, goat and aquaculture samples to deliver parentage, breed composition and inbreeding. Genomic relationship matrices are also constructed for use in genomic selection by several industry groups.
This technology is extensively utilised by the Animal and Forage Genomics Research Teams for over 50 species, including livestock, forage crops, trees, insects, birds, aquatic species (both fresh and salt water) and microbiomes.
