The Proteomics team have wide ranging expertise and capability within proteomic research.
Proteome Profiling
In proteome profiling, proteins are extracted from a large number of samples with different conditions (e.g. treated vs. non-treated).
A comparison is made between the abundance levels of all proteins identified across these samples to obtain a better understanding of what is happening to a biological system at a molecular level.
Protein Identification and PTM Characterisation
By linking acquired mass spectra with protein databases, we can identify the proteins present in samples and discover the post-translational modifications (PTMs) the proteins carry.
Peptidomics and bioactive peptides
Peptidomics focusses on characterising the small endogenous peptides in samples rather than the larger proteins. The peptides are isolated from samples by ultrafiltration using molecular weight cut-off filters.
Based on the identified peptide sequences, predictions can be made via our in-house database to see if these samples contain bioactive or taste-related peptides.
Redox Proteomics
Using our scoring system, we can compare the extent of protein modifications from redox reactions between different samples.
The redox scoring system is based on an oxidative cascade and delivers insight through the changes in consequences of the redox treatments at the molecular level.
Digestomics
Digestomic experiments put food through simulated gastro-intestinal digestion to analyse what proteins and peptides are being released during the different stages of digestion.
This allows us to track how proteins are broken down into their smaller building blocks and can be used to explore beneficial characteristics of foods.